Yu-Hui Wong 翁雨蕙 - 國立陽明交通大學神經科學研究所

YU-HuUI WONG ASSISTANT PROFESSOR

E-mail: yuhui.wong@gmail.com
TEL: +886-2-28267000 ext. 66252
Yu-Hui Wong CV

EXPERIENCE PUBLICATIONS LABORATORY

Educations

2003.09-2009.12 Ph.D. in Neuroscience, National Yang-Ming University, Taipei, Taiwan (Mentor: Ming-Ji Fann, Ph.D.)

2001.09-2003.07 M.S. in Neuroscience, National Yang-Ming University, Taipei, Taiwan

1997.04-2001.07 B.S. in Life Sciences, National Yang-Ming University, Taipei, Taiwan

Positions

2020.08-present Adjunct Assistant Professor, Department of Life Sciences and Institute of Genome Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan

2016.02-present Assistant Research Fellow, Brain Research Center, National Yang Ming Chiao Tung University, Taipei, Taiwan

2014.08-2016.01 Postdoctoral Fellow, Brain Research Center, National Yang-Ming University, Taipei, Taiwan

2010.08-2014.07 Postdoctoral Fellow, Department of Molecular and Cell Biology, University of California, Berkeley, USA (Supervisor: Mu-ming Poo, Ph.D.)

Representative Papers

- Human iPSC-derived neurons as a platform to decipher the mechanisms behind brain aging. Chao CC, Shen BW, Tzeng TY, Kung HJ, Tsai TF and Wong YH*. (2021) Biomedicines 9(11):1635; doi.org/10.3390/biomedicines9111635.
- Efficient conversion of human induced pluripotent stem cells into microglia by defined transcription factors. Chen SW, Hung YS, Fuh JL, Chen NJ, Chu YS, Chen SC, Fann MJ and Wong YH*. (2021) Stem Cell Reports 16(5): 1363-1380; doi: 10.1016/j.stemcr.2021.03.010.
- Muscle atrophy-related myotube-derived exosomal microRNA in neuronal dysfunction: Targeting both coding and long-noncoding RNAs. Yang CP#, Yang WS#, Wong YH#, Wang KH, Teng YC, Chang MH, Liao KH, Nian FS, Tsai JW, Hwang WL, Lin MW, Tzeng TY, Wang PN, Campbell M, Chen LK, Tsai TF*, Chang PC* and Kung HJ*. (2020) Aging Cell e13107; doi: 10.1111/acel.13107.
- Assessing the therapeutic potential of Graptopetalum paraguayense on Alzheimer’s disease using patient iPSC-derived neurons. Wu PC, Fann MJ, Tran T, Chen SC, Devina T, Cheng IH, Lien CC, Kao LS, Wang SJ, Fuh JL, Tzeng TT, Huang CY, Shiao YJ* and Wong YH*. (2019) Sci. Rep. 9, 19301; doi:10.1038/s41598-019-55614-9.
- GSKIP-mediated anchoring increases phosphorylation of Tau by PKA but not GSK3beta via a cAMP/PKA/GSKIP/GSK/Tau axis signaling in cerebrospinal fluid and iPS cells of Alzheimer disease. Ko HJ#, Chiou SJ#, Wong YH#, Wang YH, Lai YL, Chou CH, Wang C, Loh JK, Lieu AS, Cheng JT, Lin YT, Lu PJ, Fann MJ, Huang CY* and Hong YR*. (2019) J. Clin. Med. 8(10), pii: 1751; doi.org/10.3390/jcm810175.
- Cdk12 regulates neurogenesis and late-born neuronal migration in the developing cerebral cortex. Chen HR, Juan HC, Wong YH, Tsai JW and Fann MJ* (2017) Cereb Cortex. 27(3):2289-2302.
- Activity-dependent BDNF release via endocytic pathways is regulated by complexin and synaptotagmin-6. Wong YH, Lee CM, Xie W, Cui B and Poo MM* (2015) Proc Natl Acad Sci U S A. 112(32):E4475-84.
- Protogenin prevents premature apoptosis of rostral cephalic neural crest cells by activating the α5β1integrin. Wang YC, Juan HC, Wong YH, Kuo WC, Lu YL, Lin SF, Lu CJ and Fann MJ* (2013) Cell Death Dis. 4:e651.
- Self-amplifying autocrine actions of BDNF in axon development. Cheng PL, Song AH, Wong YH, Wang S, Zhang X and Poo MM* (2011) Proc Natl Acad Sci U S A. 108(45):18430-5.
- Protogenin defines a transition stage during embryonic neurogenesis and prevents precocious neuronal differentiation. Wong YH, Lu AC, Wang YC, Cheng HC, Chang C, Chen PH, Yu JY and Fann MJ* (2010) J Neurosci. 30(12):4428-39.
- CDK13/CDC2L5 interacts with L-type cyclins and regulates alternate splicing. Chen HH, Wong YH, Geneviere AM and Fann MJ* (2007) Biochem Biophys Res Commun. 354(3):735-40.

Patent

- Methods of producing microglia and uses thereof. (Inventor & Applicant; Appl. No: 62/872,360; US provisional; Jul 10 2019 filed)

Research Interest

Our laboratory is working on identifying the molecular mechanisms underlying how a pluripotent cell develops into a defined cell type of human brain, such as neurons, microglia, retinal ganglion cells and other neural lineages. This has allowed us to generate functional brain cells with high consistency at scale from human iPSCs, including patient-derived iPSCs or those carrying diseasespecific mutations by CRISPR/Cas9 technology, offering novel avenues for the development of human in vitro models to support research and drug discovery.

In the past four years, we used human iPSCs derived from familial and sporadic patients of Alzheimer’s disease (AD) to study disease mechanisms and to screen novel drugs for treatment. We differentiated the AD-iPSC lines into the neuronal lineage to examine whether these neurons have mature phenotypic and physiological properties, as well as AD-like biochemical features. We also established a novel protocol enabling differentiation of human microglia-like cells from hiPSCs in high purity, which is used to investigate the neuroinflammatory phenotypes of AD. In the future, AD-iPSC derived neurons and glial cells, plus 3D organoids, harboring disease properties will be used as humanized models to study the mechanisms underlying AD pathogenesis, as well as to evaluate potential drugs for AD treatment.

Research Experiences

2001.09-2009.12 Molecular and Cellular Mechanisms of Neural Development

General molecular experiments and biochemical assays (gene cloning, RT-PCR, western blot, etc.)
General cell biological experiments (mammalian cell culture, immunocytochemistry, etc.)
In vivo chick embryo manipulation (in ovo electroporation, immunohistochemistry, etc.)
Phenotype analyses of transgenic mouse embryos

2010.08-2014.07 Mechanisms and Regulation of Neurotrophin Transport and Secretion

In vitro live-cell imaging (time-lapse recording of single molecule tracking, FRET imaging, calcium imaging, and image analysis, etc.)
General cell biological experiments (primary neuronal culture, immunocytochemistry, etc.)
In vivo rat embryo manipulation (in utero electroporation, section and time-lapse imaging, immunohistochemistry, etc.)
Viral vector package (lentivirus)

2014.08-presenst Modeling of Human Neurological Disorders Using Induced Pluripotent Stem Cells

In vitro modeling of AD using patients’ iPSCs (human iPSC culture, neuron & microglia differentiation, 3D brain organoid, genome editing with CRISPR/Cas9 technology, etc.)
Evaluation of drug candidate using AD-iPSCs (in vitro AD pathological phenotype assays)