Cheng-Chang Lien 連正章 - 國立陽明交通大學神經科學研究所

Cheng-Chang Lien Distinguished Professor, INS/ Dean, College of Life Sciences

E-mail : cclien@nycu.edu.tw
Office phone: +886-2-2826-7325
Lab phone: +886-2-2826-7000 ext. 66090
FAX : +886-2-2821-5307
Website1: Laboratory of Molecular and Cellular Neurophysiology (old)
Website2: Laboratory of Molecular and Cellular Neurophysiology (new)
CV: Cheng-Chang Lien

EXPERIENCE PUBLICATIONS LABORATORY INFO LAB MEMBERS OTHER INFO

Educations

PhD from Albert-Ludwigs-Universitat Freiburg, Germany – 1998~2003

Medical doctor (MD) from China Medical University, Taiwan – 1990~1997

Positions

Since 2020.11: Dean of College of Life Sciences, National Yang Ming Chiao Tung University (previously named as National Yang-Ming University before 2021.02.01)

Since 2017.08: Distinguished Professor, National Yang Ming Chiao Tung University (previously named as National Yang-Ming University before 2021.02.01)

Since 2015.08: Professor of the Institute of Neuroscience, National Yang Ming Chiao Tung University (previously named as National Yang-Ming University before 2021.02.01)

2017.08 – 2021.01: Director of the Institute of Neuroscience, National Yang-Ming University

2015 – 2019: Visiting Professor at Charite – Medical University of Berlin, Germany

2011.02 – 2015.07: Associate Professor of the Institute of Neuroscience, National Yang-Ming University

2006.07 – 2011.02: Assistant Professor of the Institute of Neuroscience, National Yang-Ming University

2004.01 – 2006.07: Postdoc. at University of California Berkeley, Department of Molecular and Cell Biology, USA

2003.04 – 2004.02: Postdoc. at Albert-Ludwigs-Universitat Freiburg, Germany

1997.07 – 1998.06: Medical Residency, Neurology, National Taiwan University Hospital

Honors

2020/02: Honors with Qualifications for Permanent Assessment Exemption for Teachers

2017-2019: NYMU Academic Excellence Award (the same award received in 2015-2016; 2013-2014; 2011-2012)

2017-2019: Distinguished Professor, National Yang-Ming University

2017: MOST Outstanding Research Award

2016: TienTe Lee Young Scientist Research Award

2016 – 2018: Research Fellow of Alexander von Humboldt Foundation, Germany

2015 – 2016: NeuroCure Fellowship, Berlin, Germany

2012: Award of German Academic Exchange Service (DAAD) Scholarship for the research visit at the Institute for Physiology and Pathophysiology, Ruprecht-Karls-Universität Heidelberg, Germany

2007 – 2012: Teaching award for outstanding teachers in the School of Medicine, National Yang-Ming University.

2010: The Best Poster Award of 2010 TPEVGH-UST Research Grant.

2006: Award of stipend from Cold Spring Harbour for the imaging course: Imaging Structure & Function in the Nervous Systems.

2003: Doctoral thesis with the grade “summa cum laude” and research doctorate (research supervisor: Peter Jonas) from Albert-Ludwigs-Universität Freiburg, Germany.

2002: Award of stipend from Marine Biological Laboratory (Woods Hole, USA) for the “Method in Computational Neuroscience” summer course.

1998 – 2003: Award of German Academic Exchange Service (DAAD) Scholarship for the international PhD program in the Institute of Physiology, Albert-Ludwigs-Universität Freiburg, Germany.

Peer-Reviewed Papers

1. Devina T, Wong YH, Hsiao CW, Li YJ, Lien CC, Cheng IH (2022). Endoplasmic reticulum stress induces Alzheimer disease-like phenotypes in the neuron derived from the induced pluripotent stem cell with D678H mutation on amyloid precursor protein. Journal of Neurochemistry. doi: 10.1111/jnc.15687. Online ahead of print. [PDF] [PubMed]
2. Huang DF, Lee CY, Chou MY, Yang TY, Cao X, Hsiao YH, Wu RN, Lien CC, Huang YS, Huang HP, Gau SF, Huang HS (2022). Neuronal splicing regulator RBFOX3 mediates seizures via regulating Vamp1 expression preferentially in NPY-expressing GABAergic neurons. Proc Natl Acad Sci USA. 119(33):e2203632119. [PDF] [PubMed]
3. Abdulmajeed W, Wang KY, Wu JW, Ajibola MI, Cheng IH, Lien CC (2022). Connectivity and Synaptic Features of Hilar Mossy Cells and Their Effects on Granule Cell Activity Along the Hippocampal Longitudinal Axis. Journal of Physiology 600(14):3355-3381. [PDF] [PubMed] [cover1] [cover2]
4. Yen TY, Huang Y, MacLaren DAA, Schlesiger MI, Monyer H, Lien CC (2022). Inhibitory projections connecting the dentate gyri in the two hemispheres support spatial and contextual memory. Cell Reports 39(7):110831. [PDF] [PubMed] [Chinese Graphical Summary]
5. Chen WH, Lien CC, Chen CC (2022). Neuronal basis for pain-like and anxiety-like behaviors in the central nucleus of the amygdala. Pain 163(3):e463-e475. [PDF] [PubMed]
6. Feng KL, Weng JY, Chen CC, Abubaker MB, Lin HW, Charng CC, Belle JS, Tully T, Lien CC, Chiang AS (2021). Neuropeptide F inhibits dopamine neuron interference of long-term memory consolidation in Drosophila. iScience 24(12):103506. (co-corresponding author) [PDF] [PubMed]
7. Ajibola MI, Wu JW, Lien CC (2021). Hypothalamic glutamate/GABA co-transmission modulates hippocampal circuits and supports long-term potentiation. Journal of Neuroscience 41(39):8181-8196. [PDF] [PubMed]
8. Wang KY, Wu JW, Cheng JK, Chen CC, Wong WY, Averkin RG, Tamás G, Nakazawa K, Lien CC (2021). Elevation of hilar mossy cell activity suppresses hippocampal excitability and avoidance behavior. Cell Reports 36(11):109702. [PDF] [PubMed] [cover]
9. Wei YT, Wu JW, Yeh CW, Shen HC, Wu KP, Vida Imre, Lien CC (2021). Morpho-physiological properties and connectivity of vasoactive intestinal polypeptide-expressing interneurons in the mouse hippocampal dentate gyrus. Journal of Comparative Neurology 529(10):2658-2675. [PDF] [PubMed]
10. Wu PC, Fann MJ, Tran TT, Chen SC, Devina T, Cheng IH, Lien CC, Kao LS, Wang SJ, Fuh JL, Tzeng TT, Huang CY, Shiao YJ, Wong YH (2019). Assessing the therapeutic potential of Graptopetalum paraguayense on Alzheimer’s disease using patient iPSC-derived neurons. Scientific Reports 9(1): 19301. [PDF] [PubMed]
11. Hsu YT, Chang YG, Liu YC, Wang KY, Chen HM, Lee DJ, Yang SS, Tsai CH, Lien CC*, Chern YJ*. (2019). Enhanced Na+-K+-2Cl- cotransporter 1 underlies motor dysfunction in Huntington’s disease. Movement Disorders 34(6): 845-857. (*corresponding) [PDF] [PubMed]
12. Chen CY, Di Lucente J, Lin YC, Lien CC, Rogawski MA, Maezawa I, Jin LW (2018). Defective GABAergic neurotransmission in the nucleus tractus solitarius in Mecp2-null mice, a model of Rett syndrome. Neurobiology of Disease 109(Pt A): 25-32. [PDF] [PubMed]
13. Huang CY, Lien CC, Cheng CF, Yen TY, Chen CJ, Tsaur ML (2017). K+ channel Kv3.4 is essential for axon growth by limiting the influx of Ca2+ into growth cones. Journal of Neuroscience 37(17): 4433-4449. [PDF] [PubMed]
14. Kuo YL, Cheng JK, Hou WS, Chang YC, Du PH, Jian JJ, Rau RH, Yang JH, Lien CC, Tsaur ML (2017). K+ channel modulatory subunits KChIP and DPP participate in Kv4-mediated mechanical pain control. Journal of Neuroscience 37(16): 4391-4404. [PDF] [PubMed]
15. Lee CT, Kao MH, Hou WH, Wei YT, Chen CL, Lien CC (2016). Causal evidence for the role of specific GABAergic interneuron types in entorhinal recruitment of dentate granule cells. Scientific Reports 6: 36885. [PDF] [PubMed] [Supplementary Information]
16. Hou WH, Kuo N, Fang GW, Huang HS, Wu KP, Zimmer A, Cheng JK, Lien CC (2016). Wiring specificity and synaptic diversity in the mouse lateral central amygdala. Journal of Neuroscience 36(16): 4549-4563. [PDF] [PubMed]; also see Editor’s Commentary [PDF] [PDF (Chinese Ver.)]
17. Hsu TT, Lee CT, Tai MH, Lien CC (2016). Differential recruitment of dentate gyrus interneuron types by commissural versus perforant pathways. Cerebral Cortex 26(6): 2715-2727. [PDF] [PubMed] [Supplementary Information]
18. Wu CC, Lien CC, Hou WH, Chiang PM, Tsai KJ (2016). Gain of BDNF function in engrafted neural stem cells promotes the therapeutic potential for Alzheimer’s disease. Scientific Reports 6: 27358. [PDF] [PubMed]
19. Chang CP, Lee CT, Hou WS, Lin MS, Lai HL, Chien CL, Chang C, Cheng PL, Lien CC*, Chern Y* (2016). Type VI adenylyl cyclase negatively regulates GluN2B-mediated LTD and spatial reversal learning. Scientific Reports 6: 22529. (*corresponding) [PDF] [PubMed] [Supplementary Information]
20. Chiang PH, Chien TC, Chen CC, Yanagawa Y, Lien CC (2015). ASIC-dependent LTP at multiple glutamatergic synapses in amygdala network is required for fear memory. Scientific Reports 5:10143. [PDF] [PubMed] [Supporting information]
21. Lin SH, Chien YC, Chiang WW, Liu YZ, Lien CC, Chen CC (2015). Genetic mapping of ASIC4 and contrasting phenotype to ASIC1a in modulating innate fear and anxiety. European Journal of Neuroscience 41(12): 1553-1568. [PDF] [PubMed]
22. Chen WT, Hsieh YF, Huang YJ, Lin CC, Lin YT, Liu YC, Lien CC, Cheng IH (2015). G206D mutation of presenilin-1 reduces Pen2 interaction, increases Aβ42/Aβ40 ratio and elevates ER Ca2+ accumulation. Molecular Neurobiology 52(3): 1835-1849. [PDF] [PubMed]
23. Liu YC, Cheng JK, Lien CC (2014). Rapid dynamic changes of dendritic inhibition in the dentate gyrus by presynaptic activity patterns. Journal of Neuroscience 34(4): 1344-1357. [PDF] [PubMed]
24. Cheng CF, Cheng JK, Chen CY, Lien CC, Chu D, Wang SY, Tsaur ML (2014). Mirror-image pain is mediated by NGF produced from TNFα-activated satellite glia after peripheral nerve injury. Pain 155(5): 906-920. [PDF] [PubMed] [Supporting information]
25. Chan CF, Kuo TW, Weng JY, Lin YC, Chen TY, Cheng JK, Lien CC (2013). Ba2+- and bupivacaine-sensitive background K+ conductances mediate rapid EPSP attenuation in oligodendrocyte precursor cells. Journal of Physiology 591(19): 4843-4858. (an editor’s choice and the highlight by the Perspectives in the same issue) [PDF] [PubMed]; also see the perspective by Battefeld and Kole in the same issue; also selected as the Editor Choice in the same issue [PDF]
26. Wu PY, Huang YY, Chen CC, Hsu TT, Lin YC, Weng JY, Chien TC, Cheng IH, Lien CC (2013). Acid-sensing ion channel-1a is not required for normal hippocampal LTP and spatial memory. Journal of Neuroscience 33(5): 1828-1832. [PDF] [PubMed]
27. Sun YY, Lin SH, Lin HC, Hung CC, Wang CY, Lin YC, Hung KS, Lien CC, Kuan CY, Lee YH (2013). Cell type-specific dependency on the PI3K/Akt signaling pathway for the endogenous Epo and VEGF induction by baicalein in neurons versus astrocytes. PLoS ONE 8(7): e69019. [PDF] [PubMed] [Supporting information]
28. Lee YC, Durr A, Majczenko K, Huang YH, Liu YC, Lien CC, Tsai PC, Ichikawa Y, Goto J, Monin ML, Li JZ, Chung MY, Mundwiller E, Shakkottai V, Liu TT, Tesson C, Lu YC, Brice A, Tsuji S, Burmeister M, Stevanin G, Soong BW (2012). Mutations in KCND3 cause spinocerebellar ataxia type 22. Annals of Neurology 72(6): 859-869. [PDF] [PubMed] [Supporting information]
29. Majumder P, Chen YT, Bose JK, Wu CC, Cheng WC, Cheng SJ, Fang YH, Chen YL, Tsai KJ, Lien CC, Shen CK (2012). TDP-43 regulates the mammalian spinogenesis through translational repression of Rac1. Acta Neuropathologica 124(2): 231-245. [PDF] [PubMed] [Supporting information]
30. Chiang PH, Wu PY, Kuo TW, Liu YC, Chan CF, Chien TC, Cheng JK, Huang YY, Chiu CD, Lien CC (2012). GABA is depolarizing in hippocampal dentate granule cells of the adolescent and adult rats. Journal of Neuroscience 32(1): 62-67. [PDF] [PubMed]
31. Weng JY, Lin YC, Lien CC (2010). Cell type-specific expression of acid-sensing ion channels in hippocampal interneurons. Journal of Neuroscience 30(19): 6548-6558. [PDF] [PubMed] [Supporting information]
32. Lin YC, Liu YC, Huang YY, Lien CC (2010). High-density expression of Ca2+-permeable ASIC1a channels in NG2 glia of rat hippocampus. PLoS ONE 5(9): e12665. [PDF] [PubMed]
33. Chu KC, Chiu CD, Hsu TT, Hsieh YM, Huang YY, Lien CC (2010). Functional identification of an outwardly rectifying pH- and anesthetic-sensitive leak K+ conductance in hippocampal astrocytes. European Journal of Neuroscience 32(5): 725-735. [PDF] [PubMed] [Supporting information]
34. Chiang PH, Yeh WC, Lee CT, Weng JY, Huang YY, Lien CC (2010). M1-like muscarinic acetylcholine receptors regulate fast-spiking interneuron excitability in rat dentate gyrus. Neuroscience 169(1): 39-51. [PDF] [PubMed]
35. Liao CW, Lien CC (2009). Estimating intracellular Ca2+ concentrations and buffering in a dendritic inhibitory hippocampal interneuron. Neuroscience 164(4): 1701-1711. [PDF] [PubMed]
36. Lien CC, Mu Y, Vargas-Caballero M, Poo MM (2006). Visual stimuli-induced LTD of GABAergic synapses mediated by presynaptic NMDA receptors. Nature Neuroscience 9(3): 372-380. [PDF] [PubMed] [Supporting information]
37. Aponte Y, Lien CC, Reisinger E, Jonas P (2006). Hyperpolarization-activated cation channels in fast-spiking interneurons of rat hippocampus. Journal of Physiology 574(Pt 1): 229-243. [PDF] [PubMed]
38. Oliver D#, Lien CC#, Soom M, Baukrowitz T, Jonas P, Fakler B (2004). Functional conversion between A-type and delayed rectifier K+ channels by membrane lipids. Science 304(5668): 265-270. (#: equally contributing). [PDF] [PubMed] [Supporting information]
39. Lien CC, Jonas P (2003). Kv3 potassium conductance is necessary and kinetically optimized for high-frequency action potential generation in hippocampal interneurons. Journal of Neuroscience 23(6): 2058-2068. [PDF] [PubMed]
40. Lien CC, Martina M, Schultz JH, Ehmke H, Jonas P (2002). Gating, modulation, and subunit composition of voltage-gated K+ channels in dendritic inhibitory interneurones of rat hippocampus. Journal of Physiology 538(Pt 2): 405-419. [PDF] [PubMed]

Published Book Chapter

- Martina M*, Lien CC* (2018). Book Chapter: Physiological properties of hippocampal neurons. Hippocampal Microcircuits: 91-126. (*corresponding) [PDF] [Link]
- 連正章。Potassium Channels for High-Frequency Action Potential Generation in GABAergic Interneurons of Rat Hippocampus. 2003年 PhD thesis. [PDF] or PDF from https://freidok.uni-freiburg.de/data/749

Research Interest

My lab is interested in understanding brain circuits and behavior in health and disease. More recently we have become increasingly interested in neural mechanisms underlying emotion and cognition. We mainly focus on two key regions in the limbic system, that is, the hippocampus and amygdala. To establish causal relationships between brain circuits and behavior, we combine the optogenetic or chemogenetic approach with different readout methodologies including electrophysiology and calcium imaging in freely moving animals. To uncover the input-output organization of the network, we employ optogenetics-assisted circuit mapping in brain slices maintained in vitro or in intact brains. Over the past decade, we have highlighted the relevance of GABAergic circuitry in both network and cognitive functions.

One of the grand challenges in brain research is to understand the wiring principle of the brain. Over the past decade, I mainly focused on three major topics:
First, what are the hierarchy, diversity and variability of central neurons?
Second, what is the cell type-specific expression pattern of channel/receptors in the brain and what is its physiological significance?
Third, how neurotransmission at central synapses is regulated and integrated along dendrites?
Mutidisciplinary approaches are currently used in my lab including: electrophysiology such as patch-clamp technique, fast calcium imaging, high-speed voltage imaging, two-photon imaging, single-cell RT-PCR, 3D morphological reconstruction, optogenetics, neuronal modeling, molecular biology, mouse genetics, animal behavioral study, etc.

Current members

Assistant & Postdoctoral fellow
Wei-Ling Lin, Administrative Staff	Hung-Chang Shen, Postdoctoral fellow	Kai-Yi Wang, Postdoctoral fellow	Musa Iyiola Ajibola, Postdoctoral fellow	Chia-Wei Yeh, Research assistant
PhD student
Yu-Lin Lin, PhD student	Ting-Yun Yen, PhD student	Tse-Ming Chou, PhD student	Wahab Imam Abdulmajeed, PhD student	Jin-Di Chou, PhD student
Jei-Wei Wu, PhD student	Ya-Hui Wang, PhD student	Yu-Fang Chou, PhD student	Jakobus Gerick Pantouw, PhD student
Master student
Hui-Wen Huang, MS student	Yu-Rui Lee, MS student	Shih-Che Lin, MS student
Undergraduate Student
Hui-Chi Chiang, Undergraduate student	Kuan-Yu Chen, Undergraduate student	Yi-Chen Tsai, Undergraduate student

Former members

Postdoctoral fellow
Ju-Yun Weng, PhD student (graduated) Postdoctoral fellow	Yu-Chao Liu, PhD student (graduated) Postdoctoral fellow	Wen-Hsien Hou, PhD student (graduated) Postdoctoral fellow
PhD student
Ju-Yun Weng, PhD student (graduated)	Yu-Chao Liu, PhD student (graduated)	Jhih-Wei Hsu, PhD student (dropout)	Ta-Chun Chien, PhD student (dropout)	Po-Han Chiang, PhD student (graduated)
Tsan-Ting Hsu, PhD student (graduated)	Cheng-Da Lee, PhD student (graduated)	Wen-Hsien Hou, PhD student (graduated)	Chien-Chun Chen, PhD student (dropout)	Kai-Yi Wang, PhD student (graduated)
Musa Iyiola Ajibola, PhD student (graduated)	Hung-Ling Chang Chien, PhD student (dropout)	Zhu-Sen Yang, PhD student (dropout)
Master student
Wei-Chun Yeh, MS student (graduated)	Kuo-Chang Chu, MS student (graduated)	Chen-Wei Liao, MS student (graduated)	Yen-Chu Lin, MS student (graduated)	Pu-Yeh Wu, MS student (graduated)
Tzu-Wei Kuo, MS student (graduated)	Chu-Fang Chan, MS student (graduated)	Ning Kuo, MS student (graduated)	Ting-Yu Chen, MS student (graduated)	Chieh-Ju Chen, MS student (graduated)
Ting-Yun Yen, MS student (graduated)	Min-Hua Kao, MS student (graduated)	Wai-Yi Wong, MS student (graduated)	Yu-Ting Wei, MS student (graduated)	Chia-Wei Yeh, MS student (graduated)
Li-Wen Chou, MS student (graduated)	Zhu-Sen Yang, MS student (graduated)	Ssu-Min Wang, MS student (dropout)

Collaborators

Domestic

Dr. Meei-Ling Tsaur

Institute of Neuroscience, National Yang Ming Chiao Tung University

Dr. Yi-Hsuan Lee

Department and Institute of Physiology, National Yang Ming Chiao Tung University

Dr. Irene Han-Juo Cheng

Institute of Brain Science, National Yang Ming Chiao Tung University

Dr. Jen-Kun Cheng

Department of Anesthesia, Mackay Memorial Hospital

Dr. Yu-Yin Huang

Department of Anesthesia, Cheng Hsin General Hospital

Dr. Yi-Juang Chern

Institute of Biomedical Sciences, Academia Sinica

Dr. Chen, Chih-Cheng

Institute of Biomedical Sciences, Academia Sinica

Dr. Hwai-Jong Cheng

Institute of Molecular Biology, Academia Sinica

Dr. Pei-Hsin Huang

Department of Pathology and Graduate Institute of Pathology, National Taiwan University

International

Dr. Imre Vida

Institute of Integrative Neuroanatomy, Charité – Universitätsmedizin Berlin

Dr. Gábor Tamás

University of Szeged, Department of Physiology

Dr. Hannah Monyer

Department of Clinical Neurobiology, University of Heidelberg

Dr. Marco Capogna

Department of Biomedicine, Aarhus University

Dr. Francesco Ferraguti

Department of Pharmacology, Medical University of Innsbruck

Dr. Chou-Long Huang

Department of Internal Medicine, College of Medicine, University of Iowa Caver

Dr. Kazutoshi Nakazawa

Department of Neurobiology, University of Alabama Birmingham

Media Coverage

(Video) Lien Lab Introduction

(Video) Life in Lien Lab

Media Coverage

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連正章老師訪談《陽明大學Investigator》

Contact Us

E-mail: cclien@ym.edu.tw or cclien@nycu.edu.tw

Office phone: +886-2-2826-7325

Lab phone: +886-2-2826-7000 ext. 66090

Fax: +886-2-2821-5307

Mailing address: No.155, Sec. 2, Linong St., Beitou Dist., Taipei city 112, Taiwan.
Institute of Neuroscience, National Yang Ming Chiao Tung University

Directions: Room 750, Library, Information and Research Building